Abstract Title

Antibacterial activity of cyanobacterial extracts against Legionella spp.

Start Date

24-5-2022 5:45 PM

End Date

24-5-2022 7:00 PM

Abstract

Cyanobacteria are recognized sources of natural compounds with pharmaceutical interest, namely antimicrobial activity. Several studies have shown the inhibitory effect of cyanobacteria against the most common bacterial pathogens. However, the bioactivity against Legionella pneumophila was never reported. L.pneumophila is ubiquitous in water environments and causes respiratory infections through water-air transmission. A rise in Legionella outbreaks might be expected, considering that climate changes will ex-acerbate water-borne diseases. In this work, we evaluated the antibacterial potential of 25 freshwater cyanobacteria from ESSACC collection, against clinical/environmental L. pneumophila strains isolated from outbreaks (cooling towers systems). Cyanobacterial biomass was extracted with n-hexane, dicloromethane:methanol (2:1), methanol 70%, and water and extracts were dried and dissolved in DMSO (25%). Disk Diffusion method was adapted to Legionella growth using 1 McFarland suspensions in BCYE plates. Levofloxacin (10 µg) and DMSO (25%) were used as positive/negative controls, respectively. Methanolic extracts from Dolichospermum flos-aquae, Limnothrix redekei, Microcystis aeruginosa and Planktothrix agardhii (LMECYA 165, 145, 257 and 127) induced inhibition zones ≥10 mm, demonstrating their antibacterial activity against L. pneumophila. These results encourage us to further investigate the potentiality of cyanobacteria as natural sources of antibiotics and/or water disinfectants, to overcome the occurrence of pathogenic Legionella in water environments.

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May 24th, 5:45 PM May 24th, 7:00 PM

Antibacterial activity of cyanobacterial extracts against Legionella spp.

Cyanobacteria are recognized sources of natural compounds with pharmaceutical interest, namely antimicrobial activity. Several studies have shown the inhibitory effect of cyanobacteria against the most common bacterial pathogens. However, the bioactivity against Legionella pneumophila was never reported. L.pneumophila is ubiquitous in water environments and causes respiratory infections through water-air transmission. A rise in Legionella outbreaks might be expected, considering that climate changes will ex-acerbate water-borne diseases. In this work, we evaluated the antibacterial potential of 25 freshwater cyanobacteria from ESSACC collection, against clinical/environmental L. pneumophila strains isolated from outbreaks (cooling towers systems). Cyanobacterial biomass was extracted with n-hexane, dicloromethane:methanol (2:1), methanol 70%, and water and extracts were dried and dissolved in DMSO (25%). Disk Diffusion method was adapted to Legionella growth using 1 McFarland suspensions in BCYE plates. Levofloxacin (10 µg) and DMSO (25%) were used as positive/negative controls, respectively. Methanolic extracts from Dolichospermum flos-aquae, Limnothrix redekei, Microcystis aeruginosa and Planktothrix agardhii (LMECYA 165, 145, 257 and 127) induced inhibition zones ≥10 mm, demonstrating their antibacterial activity against L. pneumophila. These results encourage us to further investigate the potentiality of cyanobacteria as natural sources of antibiotics and/or water disinfectants, to overcome the occurrence of pathogenic Legionella in water environments.