Abstract Title

Modification of the freshwater Aphanizomenon flos-aquae cyanobacterium proteome at cyanophage infection

Start Date

26-5-2022 10:00 AM

End Date

26-5-2022 10:15 AM

Abstract

Marine cyanophages can maintain the metabolism of infected cyanobacterial cells and switch metabolic processes to multiply progeny viruses efficiently but there is no sufficient knowledge on whether freshwater cyanophages can control biochemical pathways of the host and if so, how this is done. Preliminary studies of Aphanizomenon flos‑aquae phage infection suggested a modification of carbon metabolism by the impact on gene expression and the level of ATP and NADPH. In the current work the proteomes of A. flos‑aquae cells infected and uninfected with vb‑AphaS‑CL131 virus were compared using 2D‑DIGE and LS‑MSn to investigate proteomic alterations caused by the infection.

The level of proteins involved in carbon dioxide assimilation has been significantly reduced in the infected A. flos‑aquae population in favour of the stimulation of the pentose‑phosphate pathway. The expression of proteins related to glycolysis as well as the synthesis of nucleotides, amino acids, pigments and chaperones was also stimulated compared to the uninfected control cultures. Therefore, these alterations indicate infection-induced translational alterations of the host cells, most likely to enhance the production of new virions. This suggests that freshwater cyanophages may modify the host's metabolism.

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May 26th, 10:00 AM May 26th, 10:15 AM

Modification of the freshwater Aphanizomenon flos-aquae cyanobacterium proteome at cyanophage infection

Marine cyanophages can maintain the metabolism of infected cyanobacterial cells and switch metabolic processes to multiply progeny viruses efficiently but there is no sufficient knowledge on whether freshwater cyanophages can control biochemical pathways of the host and if so, how this is done. Preliminary studies of Aphanizomenon flos‑aquae phage infection suggested a modification of carbon metabolism by the impact on gene expression and the level of ATP and NADPH. In the current work the proteomes of A. flos‑aquae cells infected and uninfected with vb‑AphaS‑CL131 virus were compared using 2D‑DIGE and LS‑MSn to investigate proteomic alterations caused by the infection.

The level of proteins involved in carbon dioxide assimilation has been significantly reduced in the infected A. flos‑aquae population in favour of the stimulation of the pentose‑phosphate pathway. The expression of proteins related to glycolysis as well as the synthesis of nucleotides, amino acids, pigments and chaperones was also stimulated compared to the uninfected control cultures. Therefore, these alterations indicate infection-induced translational alterations of the host cells, most likely to enhance the production of new virions. This suggests that freshwater cyanophages may modify the host's metabolism.