Abstract Title
Investigation of CyanoMetabolites in Brazilian cyanobacteria using LC-MS-QTOF: distribution in different cultured strains, bloom samples and physiological studies
Start Date
24-5-2022 4:45 PM
End Date
24-5-2022 5:00 PM
Abstract
The diversity of Brazilian cyanobacterial secondary metabolites has been investigated by our group by mass spectrometry and informatic tools for structural identification. We used both cultured strains and samples of blooms from water reservoirs. This study aimed to evaluate the metabolomic profiles of potentially toxic and non-toxic cyanobacteria by high-resolution mass spectrometry. Samples were analyzed by Ultra Performance Liquid Chromatography and quadrupole time of flight mass spectrometry (UPLC-QTOF) and data were assessed to verify changes in metabolomic profiles. Matrices were used to generate principal component analysis biplots, and molecular networks were obtained using the Global Natural Products platform. Most strains showed significant changes in their metabolomic profiles after comparison. The identified compounds included aeruginosins, spumigins, cyanopeptolins, microginins, namalides, pseudospumigins, anabaenopeptins, mycosporine-like amino acids, nodularins and microcystins. Data showed that cyanobacteria display broad metabolic plasticity among species and genera, including the synthesis and differential expression of a variety of secondary metabolites. CyanoMetabolite identification and dereplication using Metaboanalyst 4.0 integrated with CyanoMetDB (available at 10.1016/j.watres.2021.117017) are a powerful tool for identification and screening of natural products in cyanobacteria. The different cyanobacterial genera from diverse Brazilian biomes and environments are prolific sources of cyanopeptides and other classes of metabolites, including cyanotoxins.
Investigation of CyanoMetabolites in Brazilian cyanobacteria using LC-MS-QTOF: distribution in different cultured strains, bloom samples and physiological studies
The diversity of Brazilian cyanobacterial secondary metabolites has been investigated by our group by mass spectrometry and informatic tools for structural identification. We used both cultured strains and samples of blooms from water reservoirs. This study aimed to evaluate the metabolomic profiles of potentially toxic and non-toxic cyanobacteria by high-resolution mass spectrometry. Samples were analyzed by Ultra Performance Liquid Chromatography and quadrupole time of flight mass spectrometry (UPLC-QTOF) and data were assessed to verify changes in metabolomic profiles. Matrices were used to generate principal component analysis biplots, and molecular networks were obtained using the Global Natural Products platform. Most strains showed significant changes in their metabolomic profiles after comparison. The identified compounds included aeruginosins, spumigins, cyanopeptolins, microginins, namalides, pseudospumigins, anabaenopeptins, mycosporine-like amino acids, nodularins and microcystins. Data showed that cyanobacteria display broad metabolic plasticity among species and genera, including the synthesis and differential expression of a variety of secondary metabolites. CyanoMetabolite identification and dereplication using Metaboanalyst 4.0 integrated with CyanoMetDB (available at 10.1016/j.watres.2021.117017) are a powerful tool for identification and screening of natural products in cyanobacteria. The different cyanobacterial genera from diverse Brazilian biomes and environments are prolific sources of cyanopeptides and other classes of metabolites, including cyanotoxins.