Identification of Cyanometabolites and Toxicity Assessment of Cyanobacteria Isolates from Chosen Polish Eutrophic Waters and Polar Glaciers

Lukasz Wejnerowski, Department of Hydrobiology, Institute of Environmental Biology, Faculty of Biology, Adam Mickiewicz University, Poznań, PolandFollow
Tamara Dulić, Biochemistry, Faculty of Science and Engineering, Åbo Akademi University, Turku, FinlandFollow
Sultana Akter, Molecular Biotechnology and Diagnostics, Department of Biochemistry, University of Turku, Turku, FinlandFollow
Anna Czerepska, Department of Hydrobiology, Institute of Environmental Biology, Faculty of Biology, Adam Mickiewicz University, Poznań, PolandFollow
Filip Pniewski, Institute of Oceanography, University of Gdańsk, Gdańsk, PolandFollow
Michał Rybak, Department of Water Protection, Institute of Environmental Biology, Faculty of Biology, Adam Mickiewicz University, Poznań, PolandFollow
Zorica Svirčev, Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Novi Sad, SerbiaFollow
Krzysztof Zawierucha, Department of Animal Taxonomy and Ecology, Faculty of Biology, Adam Mickiewicz University, Poznań, PolandFollow
Ewa Poniecka, Department of Environmental Microbiology and Biotechnology, Faculty of Biology, University of Warsaw, Warsaw, PolandFollow
Katarzyna Taylor, Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznań, PolandFollow
Agnieszka Piasecka, Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznań, PolandFollow
Marcin Krzysztof Dziuba, Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, USAFollow
Jussi Meriluoto, Biochemistry, Faculty of Science and Engineering, Åbo Akademi University, Turku, FinlandFollow

Start Date

24-5-2022 11:15 AM

End Date

24-5-2022 11:30 AM

Abstract

Cyanobacteria have the ability to produce various types of bioactive compounds. Some of them can exert toxic effects on aquatic fauna, including daphnids, which are sensitive organisms that respond fast to toxins. Therefore, these crustaceans are useful for aquatic toxicity assessment. In this study, several dozen cyanobacterial strains isolated from different types of water bodies were analyzed for the presence of anabaenopeptins (APs), anatoxin-a (ATX-a), β-methylamino-L-alanine (BMAA), cylindrospermopsin (CYN), microcystins (MCs), nodularin (NOD), saxitoxin (STX) using immunoassay and chromatographic techniques. We also investigated the toxicity of extracts and filtrates from 40-days-old cyanobacterial cultures using biotests with D. magna and D. pulicaria.

Analytical techniques revealed the presence of the cyanometabolites studied in isolates of Aphanizomenon (APs, BMAA, CYN, STX), Planktothrix (APs, MCs), Cuspidothrix (ATX-a, CYN) and Limnothrix (BMAA). Polar strains were free of examined compounds. Biotests revealed a reduction in Daphnia survival in response to some extracts and filtrates. It was also found in the case of one polar strain. However, there was no clear pattern indicating that extracts/filtrates from strains with identified toxic compounds reduced Daphnia survival more than other strains. This suggests that other yet unknown toxic compounds are at play.

This study was financed by the Polish National Agency for Academic Exchange (project no. PPN/BEK/2020/1/00241), and partially by the National Science Centre in Poland (project no. UMO-2020/39/D/NZ8/02436).

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May 24th, 11:15 AM May 24th, 11:30 AM

Identification of Cyanometabolites and Toxicity Assessment of Cyanobacteria Isolates from Chosen Polish Eutrophic Waters and Polar Glaciers

Cyanobacteria have the ability to produce various types of bioactive compounds. Some of them can exert toxic effects on aquatic fauna, including daphnids, which are sensitive organisms that respond fast to toxins. Therefore, these crustaceans are useful for aquatic toxicity assessment. In this study, several dozen cyanobacterial strains isolated from different types of water bodies were analyzed for the presence of anabaenopeptins (APs), anatoxin-a (ATX-a), β-methylamino-L-alanine (BMAA), cylindrospermopsin (CYN), microcystins (MCs), nodularin (NOD), saxitoxin (STX) using immunoassay and chromatographic techniques. We also investigated the toxicity of extracts and filtrates from 40-days-old cyanobacterial cultures using biotests with D. magna and D. pulicaria.

Analytical techniques revealed the presence of the cyanometabolites studied in isolates of Aphanizomenon (APs, BMAA, CYN, STX), Planktothrix (APs, MCs), Cuspidothrix (ATX-a, CYN) and Limnothrix (BMAA). Polar strains were free of examined compounds. Biotests revealed a reduction in Daphnia survival in response to some extracts and filtrates. It was also found in the case of one polar strain. However, there was no clear pattern indicating that extracts/filtrates from strains with identified toxic compounds reduced Daphnia survival more than other strains. This suggests that other yet unknown toxic compounds are at play.

This study was financed by the Polish National Agency for Academic Exchange (project no. PPN/BEK/2020/1/00241), and partially by the National Science Centre in Poland (project no. UMO-2020/39/D/NZ8/02436).