Cyanobacterial toxins in recreational and intake waters using a targeted UPLC/MS/MS method
Start Date
23-5-2022 12:00 PM
End Date
23-5-2022 12:15 PM
Abstract
Liquid Chromatography/Tandem-Mass Spectrometry (LC/MS/MS) is a powerful tool for the analysis of various analytes in a wide variety of matrices. What is especially attractive about LC/MS/MS is its sensitivity and selectivity. Various cyanobacterial algae that produce toxins, microcystins being one of the more well-known, represent an emerging class of algal toxins of concern to both the recreational and drinking water industry. In this paper we investigate the use of smaller column packing (sub 2μm particles) to monitor for many of these toxins using a generic gradient method. Specifically, microcystins, anatoxin-a, cylindrospermopsin as well as newer toxins, such as euglenophycin, anabaenopeptins and micropeptins using Ultra-Performance Liquid Chromatography (UPLC®) combined with tandem mass spectrometry will also be tested. We investigated the analysis of a wide variety of freshwater samples from throughout the United States and cultured strains from the lab. Examples showing additional toxins detected in freshwater samples will be presented including data from the recent blooms in the North Carolina, Ecuador and Ohio. Comparison of blooms from a similar source over several years will also be discussed with similarities shown in the targeted analysis over that time.
Cyanobacterial toxins in recreational and intake waters using a targeted UPLC/MS/MS method
Liquid Chromatography/Tandem-Mass Spectrometry (LC/MS/MS) is a powerful tool for the analysis of various analytes in a wide variety of matrices. What is especially attractive about LC/MS/MS is its sensitivity and selectivity. Various cyanobacterial algae that produce toxins, microcystins being one of the more well-known, represent an emerging class of algal toxins of concern to both the recreational and drinking water industry. In this paper we investigate the use of smaller column packing (sub 2μm particles) to monitor for many of these toxins using a generic gradient method. Specifically, microcystins, anatoxin-a, cylindrospermopsin as well as newer toxins, such as euglenophycin, anabaenopeptins and micropeptins using Ultra-Performance Liquid Chromatography (UPLC®) combined with tandem mass spectrometry will also be tested. We investigated the analysis of a wide variety of freshwater samples from throughout the United States and cultured strains from the lab. Examples showing additional toxins detected in freshwater samples will be presented including data from the recent blooms in the North Carolina, Ecuador and Ohio. Comparison of blooms from a similar source over several years will also be discussed with similarities shown in the targeted analysis over that time.