Honors Projects
Abstract
eDNA, or environmental DNA, is found free-existing in any environment. It can be extracted from its environment and used to identify organisms that were recently in the area. In recent years, eDNA metabarcoding has been used to help examine communities for overall species presence and biodiversity. This capstone sought to create a mock proposal for an eDNA survey of Gulf of Mexico elasmobranch and Selachii species due to their overall decline in numbers. Additionally, general and discriminatory PCR primers were created in-silico and assessed for initial quality. This capstone documents the feasibility of an eDNA study, constructing the necessary primers, and the future of using ancient eDNA with modern eDNA to establish thorough baselines for conservation.
Department
Biological Sciences
Major
Marine and Aquatic Biology
First Advisor
Christopher Ward
First Advisor Department
Biological Sciences
Second Advisor
Margaret Yacobucci
Second Advisor Department
Geology
Publication Date
Spring 4-22-2024
Repository Citation
Lima, Isabella, "eDNA Metabarcoding and the Construction of Primers for the Identification of Gulf of Mexico Elasmobranchs and Selachii" (2024). Honors Projects. 1000.
https://scholarworks.bgsu.edu/honorsprojects/1000
Included in
Bioinformatics Commons, Genomics Commons, Marine Biology Commons