The muscle activity of the Tibialis Anterior, Gastrocnemius Medialis, Adductor Longus, Vastus Medialis, Tensor Fasciae Latae and Bíceps Femoris muscles in the typical water polo eggbeater kick were evaluated for four national level water polo players. The surface electromyographic (EMG) signals were collected during an intermittent protocol in which the basic position with arms up and horizontal position variants were performed. Descriptive statistics and inferential statistics (including ANOVA) were calculated using the p < .05 significance level. We observed that different muscles have different relevance during the eggbeater kick cycle, changing as a function of the movement phase. When comparing the studied three variations of eggbeater kick, differences were noticed within each phase: the outward phase and the power phase seem to impose higher muscular activity than the recovery phase for most of the studied muscles. Considering the eggbeater kick variations, arms up seems to determine higher EMG values while the basic position and the horizontal position seams to show similar EMG values.