Abstract Title

Development of a Dietary Supplement Reference Material for Multiple Classes of Cyanobacterial Toxins

Start Date

24-5-2022 5:45 PM

End Date

24-5-2022 7:00 PM

Abstract

Some algal dietary supplements are prepared using cyanobacterial biomass harvested from lakes and ponds, where multiple algal species can co-occur. This can result in supplement products containing cyanotoxins that present a risk for human health. Reference materials are therefore required to assist in the development, validation and quality control of analytical methods used for raw material and product testing for both research and regulatory purposes.

A dietary supplement certified reference material (CRM), representative in composition of an Aphanizomenon dietary supplement, was prepared by blending biomass of a variety of toxic cyanobacterial species with a typically-consumed non-toxic species (Aphanizomenon sp.). The toxin profile of this material includes microcystins, nodularins, anatoxins, cylindrospermopsins and saxitoxins. An exhaustive extraction method with a multi-analyte LC–MS/MS method was optimized and validated for homogeneity and stability evaluation. Methods for specific toxin classes will be used to assign certified values for analytes where equivalent calibration solution CRMs are available. Strategies for matrix effect compensation in LC–MS include isotope dilution and standard addition. Finally, untargeted high resolution mass spectrometry methods will be used to characterize the rich profile of non-certified cyanotoxins and other cyanobacterial secondary metabolites present.

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May 24th, 5:45 PM May 24th, 7:00 PM

Development of a Dietary Supplement Reference Material for Multiple Classes of Cyanobacterial Toxins

Some algal dietary supplements are prepared using cyanobacterial biomass harvested from lakes and ponds, where multiple algal species can co-occur. This can result in supplement products containing cyanotoxins that present a risk for human health. Reference materials are therefore required to assist in the development, validation and quality control of analytical methods used for raw material and product testing for both research and regulatory purposes.

A dietary supplement certified reference material (CRM), representative in composition of an Aphanizomenon dietary supplement, was prepared by blending biomass of a variety of toxic cyanobacterial species with a typically-consumed non-toxic species (Aphanizomenon sp.). The toxin profile of this material includes microcystins, nodularins, anatoxins, cylindrospermopsins and saxitoxins. An exhaustive extraction method with a multi-analyte LC–MS/MS method was optimized and validated for homogeneity and stability evaluation. Methods for specific toxin classes will be used to assign certified values for analytes where equivalent calibration solution CRMs are available. Strategies for matrix effect compensation in LC–MS include isotope dilution and standard addition. Finally, untargeted high resolution mass spectrometry methods will be used to characterize the rich profile of non-certified cyanotoxins and other cyanobacterial secondary metabolites present.