Honors Projects

Amy Thomas

Abstract

Heparin and protamine have been found neutralize each other such that the anticoagulant effects of both compounds are not observed. In the present study, the effects of histone and glycosaminoglycans on the formation and degradation of the 1°Xaα-ATIII complex to the 3°Xaβ-ATIII complex are explored. In the presence of histone, there is a dramatic drop in the levels of 1°Xa-ATIII complexes and an increase in the levels of 3°Xa-ATIII complexes, measured relative to the control, indicating the effect of histone to promote the degradation of the 1°Xa-ATIII complexes. Heparin alone moderately reduces levels of the 1°Xa-ATIII complexes and the 3°Xaα-ATIII complex, while having no appreciable effect on the 3°Xaβ-ATIII complex. When mixed, histone and heparin return the levels of the 1°Xa-ATIII complexes to near control levels while slightly decreasing the levels of 3°Xa-ATIII complexes, indicating an inhibition of the histone effect by heparin. In the presence of chondroitin sulfate A, histone slightly lowers the 1°Xa-ATIII complexes while having no appreciable effect on the 3°Xa-ATIII complexes, indicating a slight inhibition of the histone effect, although it does not have as great an inhibitory effect as heparin does. Dermatan sulfate (CSB) and chondroitin sulfate C, when individually mixed with histone, increase levels of the 1°Xa-ATIII complexes while decreasing the levels of 3°Xa-ATIII complexes, indicating that both compounds inhibit degradation of the 1°Xa-ATIII complexes. Additionally, the data suggest that dermatan sulfate and chondroitin sulfate C exhibit a higher inhibitory effect on histone than heparin.

Major

Chemistry

First Advisor

Arthur S. Brecher

First Advisor Department

Chemistry

Second Advisor

John T. Chen

Second Advisor Department

Mathematics and Statistics

Publication Date

Fall 12-12-2013

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