Cd2+-induced Conformational Change Of A Synthetic Metallopeptide: Slow Metal Binding Followed By A Slower Conformational Change

Author(s)

Madhumita Mukherjee
Xianchun Zhu
Michael Y. Ogawa, Bowling Green State UniversityFollow

Document Type

Article

Abstract

A two-stranded a-helical coiled coil was prepared having a Cys(4) metal-binding site within its hydrophobic interior. The addition of Cd2+ results in the incorporation of 2 equiv of metal ion, which is accompanied by a conformational change of the peptide, as observed by circular dichroism (CID) spectroscopy. Isothermal titration calorimetry (ITC) shows that the addition of Cd2+ is accompanied by two thermodynamic events. A comparison of the time dependence of the ITC behavior with those of the UV absorption and CID behavior allows the assignment of these events to a preliminary endothermic metal-binding step followed by a slower exothermic conformational change.

Copyright Statement

Publisher PDF

Repository Citation

Mukherjee, Madhumita; Zhu, Xianchun; and Ogawa, Michael Y., "Cd2+-induced Conformational Change Of A Synthetic Metallopeptide: Slow Metal Binding Followed By A Slower Conformational Change" (2008). Chemistry Faculty Publications. 175.
https://scholarworks.bgsu.edu/chem_pub/175

Publication Date

6-2008

Publication Title

Inorganic Chemistry

DOI

https://doi.org/10.1021/ic702370k

Start Page No.

4430

End Page No.

4432