Generating New Specific Rna Interaction Interfaces Using C-loops

Author(s)

Kirill A. Afonin
Neocles B. Leontis, Bowling Green State UniversityFollow

Document Type

Article

Abstract

New RNA interaction interfaces are reported for designing RNA modules for directional supramolecular self-assembly. The new interfaces are generated from existing ones by inserting C-loops between the interaction motifs that mediate supramolecular assembly. C-Loops are new modular motifs recently identified in crystal structures that increase the helical twist of RNA helices in which they are inserted and thus reduce the distance between pairs of loop or loop-receptor motifs from 11 to 9 base-stacking layers while maintaining correct orientation for binding to cognate interaction interfaces. Binding specificities of C-loop-containing molecules for cognate molecules that also have inserted C-loops were found to range up to 20-fold. Binding affinities for most C-loop-containing molecules were generally equal or higher than those for the parent molecules lacking C-loops.

Copyright Statement

Publisher PDF

Repository Citation

Afonin, Kirill A. and Leontis, Neocles B., "Generating New Specific Rna Interaction Interfaces Using C-loops" (2006). Chemistry Faculty Publications. 147.
https://scholarworks.bgsu.edu/chem_pub/147

Publication Date

12-2006

Publication Title

Journal Of The American Chemical Society

DOI

https://doi.org/10.1021/ja064289h

Start Page No.

16131

End Page No.

16137