Chemistry Faculty Publications

Document Type

Article

Abstract

A two-stranded a-helical coiled coil was prepared having a Cys(4) metal-binding site within its hydrophobic interior. The addition of Cd2+ results in the incorporation of 2 equiv of metal ion, which is accompanied by a conformational change of the peptide, as observed by circular dichroism (CID) spectroscopy. Isothermal titration calorimetry (ITC) shows that the addition of Cd2+ is accompanied by two thermodynamic events. A comparison of the time dependence of the ITC behavior with those of the UV absorption and CID behavior allows the assignment of these events to a preliminary endothermic metal-binding step followed by a slower exothermic conformational change.

Publication Date

6-2008

Publication Title

Inorganic Chemistry

Volume

47

Issue

11

Start Page No.

4430

End Page No.

4432

DOI

10.1021/ic702370k

Included in

Chemistry Commons

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