Chemistry Faculty Publications
Document Type
Article
Abstract
New RNA interaction interfaces are reported for designing RNA modules for directional supramolecular self-assembly. The new interfaces are generated from existing ones by inserting C-loops between the interaction motifs that mediate supramolecular assembly. C-Loops are new modular motifs recently identified in crystal structures that increase the helical twist of RNA helices in which they are inserted and thus reduce the distance between pairs of loop or loop-receptor motifs from 11 to 9 base-stacking layers while maintaining correct orientation for binding to cognate interaction interfaces. Binding specificities of C-loop-containing molecules for cognate molecules that also have inserted C-loops were found to range up to 20-fold. Binding affinities for most C-loop-containing molecules were generally equal or higher than those for the parent molecules lacking C-loops.
Copyright Statement
Publisher PDF
Repository Citation
Afonin, Kirill A. and Leontis, Neocles B., "Generating New Specific Rna Interaction Interfaces Using C-loops" (2006). Chemistry Faculty Publications. 147.
https://scholarworks.bgsu.edu/chem_pub/147
Publication Date
12-2006
Publication Title
Journal Of The American Chemical Society
DOI
https://doi.org/10.1021/ja064289h
Start Page No.
16131
End Page No.
16137