The optimized synthesis and purification methodology for an intermolecular pyrophosphate sensor scaffold molecule was performed: high performance liquid chromatography afforded the scaffold in excellent purity and yield. The applications of the sensor assembly in the quantitative polymerase chain reaction were discussed, and preliminary results show that
Dr. Pavel Anzenbacher
First Advisor Department
Dr. Julia Halo
Second Advisor Department
Gevedon, Ethan, "The Synthesis and Purification Methodology of an Intermolecular Pyrophosphate Sensor: Applications for the Quantitative Polymerase Chain Reaction" (2022). Honors Projects. 688.