Given the growing issue in healthcare of antibiotic resistance, effective and safe alternative treatment methods are required. One of these possible alternative treatment methods is bacteriotoxins including bacteriocins and tailocins. The focus of this study is a bacteriotoxin from Burkholderia cenocepacia (ATCC 25608), which was induced for toxin using a modified UV light induction procedure and tested against a variety of Pseudomonas and Burkholderia for its killing capacity. Various other pathogenic strains were then induced with UV light and tested. The results showed that the toxin from ATCC 25608 was very effective against most of the Burkholderia tested and warrants further study for possible clinical use. The pathogenic Burkholderia strains tested, however, did not produce any affective toxin against the indicator cells used in the study. The study also indicated that storage of toxin is most effective at 4°C, whereas storage in glycerol at -20°C appears to limit the toxin’s effectiveness.
Dr. Ray Larsen
First Advisor Department
Dr. Joseph Furgal
Second Advisor Department
Walny, Kyle, "Antimicrobial Activity of Bacterial Virus Components: An Empirical Investigation of the Killing Capacity of Toxins from Burkholderia" (2021). Honors Projects. 659.