Heparin is a common anticoagulant used in hospitals that has been found to promote the interaction of human factor IXa with Anti-Thrombin III, creating a [IXa-ATIII] complex when put through electrophoresis. Protamine Sulfate has been found to inhibit such interactions. Therefore, this project will entail the use of four different human factors that are commonly found in the blood cascading system, Human Factors IXa, Xa, XIa, and IIa, to observe their interaction with Anti-Thrombin III. These human factors will be used in correspondence with two polyamines, Spermine and Spermidine, to see if these polyamines act more like Heparin and promote human factor and anti-thrombin interaction or like Protamine Sulfate and inhibit such formations of complexes.
Dr. Arthur Brecher
First Advisor Department
Dr. Daniel Pavuk
Second Advisor Department
Menicucci, Caitlyn, "The Effects of Spermine and Spermidine on the Interactions of Human Factors IXa, Xa, XIa, and IIa wi" (2013). Honors Projects. 38.