Chemistry Faculty Publications
Document Type
Article
Abstract
We present a study of excited-state dynamics of two flavin cofactors: flavin-adenine dinucleotide (FAD) and flavin-mononucleotide (FMN). We used femtosecond mid-R transient absorption spectroscopy to study the effect of FAD conformation oil its excited-state behavior. The conformation of FAD was modulated by changing the solvent polarity: in D(2)O, FAD is present predominantly in the "stacked" conformation, in which flavin and adenine moieties are in close proximity to each other, whereas the increased amount of DMSO led to an increased amount of the "open" conformer. FMN served as a model system which lacks adenine. We found that the "stacked" conformer undergoes ail intramolecular photoinduced electron transfer from adenine to flavin with the forward electron transfer rate of k(f) = 1.9 . 10(11) s(-1) and the geminate recombination rate of k(b) = 1.1 . 10(11) s(-1). In the case of the "open" conformer, no intramolecular electron transfer was observed.
Copyright Statement
Publisher PDF
Repository Citation
Li, Guifeng and Glusac, Ksenija D., "The Role Of Adenine In Fast Excited-state Deactivation Of Fad: A Femtosecond Mid-ir Transient Absorption Study" (2009). Chemistry Faculty Publications. 71.
https://scholarworks.bgsu.edu/chem_pub/71
Publication Date
7-2009
Publication Title
Journal Of Physical Chemistry B
DOI
https://doi.org/10.1021/jp905020u
Start Page No.
9059
End Page No.
9061