Biological Sciences Faculty Publications
Title
Unraveling the Determinants of Protrusion Formation
Document Type
Article
Abstract
A COMPUTERIZED MORPHOMETRIC CLASSIFICATION TECHNIQUE BASED ON LATENT FACTORS REVEALS MAJOR PROTRUSION CLASSES: factors 4, 5, and 7. Previous work showed that factor 4 represented filopodia, 5 the distribution of lamellar cytoplasm, and 7 a blunt protrusion. We explore the relationship of focal contact (FC) characteristics and their integrated actin cables to factors values. The results show that FC maturation/cytoskeletal integration affects factor 5, because FC elongation/integration was correlated with its values. On the contrary, 7 values decreased with maturation, so cable or FC size or their integration must be restricted to form these protrusions. Where integration did occur, the cables showed distinctive size and orientation, as indicated by correlation of 7 values with FC shape. Results obtained with myosin inhibitors support the interpretation that a central, isometric, contractile network puts constraints on both factor 5 and 7 protrusions. We conclude that cells establish functional domains by rearranging the cytoskeleton.
Copyright Statement
Publisher PDF
Publisher's Statement
Availability via databases maintained by the United States National Library of Medicine.
Repository Citation
Cayer, Marilyn L.; Heckman, Carol A.; Varghese, Mita; Gorsevski, Peter; and Boudreau, Nancy S., "Unraveling the Determinants of Protrusion Formation" (2012). Biological Sciences Faculty Publications. 33.
https://scholarworks.bgsu.edu/bio_sci_pub/33
Publication Date
2012
Publication Title
International Journal of Cell Biology
DOI
https://doi.org/10.1155/2012/402916