Chemistry Faculty Publications
Document Type
Article
Abstract
Combining atomic force microscopy and fluorescence resonance energy transfer spectroscopy (AFM-FRET), we have developed a single-molecule AFM-FRET nanoscopy approach capable of effectively pinpointing and mechanically manipulating a targeted dye-labeled single protein in a large sampling area and simultaneously monitoring the conformational changes of the targeted protein by recording single-molecule FRET time trajectories. We have further demonstrated an application of using this nanoscopy on manipulation of single-molecule protein conformation and simultaneous single-molecule FRET measurement of a Cy3-Cy5-labeled kinase enzyme, HPPK (6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase). By analyzing time-resolved FRET trajectories and correlated AFM force pulling curves of the targeted single-molecule enzyme, we are able to observe the protein conformational changes of a specific coordination by AFM mechanic force pulling.
Copyright Statement
Publisher PDF
Repository Citation
He, Yufan; Lu, Maolin; Cao, Jin; and Lu, H. Peter, "Manipulating Protein Conformations By Single-molecule Afm-fret Nanoscopy" (2012). Chemistry Faculty Publications. 133.
https://scholarworks.bgsu.edu/chem_pub/133
Publication Date
2-2012
Publication Title
Acs Nano
DOI
https://doi.org/10.1021/nn2038669
Start Page No.
1221
End Page No.
1229