Biological Sciences Faculty Publications

Title

Comparisons of Protocols for Decontamination of Environmental Ice Samples for Biological and Molecular Examinations

Document Type

Article

Abstract

Drilling and laboratory manipulations of glacial ice cores introduce contemporary microbes and biomolecules onto the cores. We report herein a systematic comparative study of several decontamination protocols. Only treatment with 5% sodium hypochlorite eliminated all external contaminating microbes and nucleic acids while maintaining the integrity of those within the cores.

Publisher's Statement

Availability via databases maintained by the United States National Library of Medicine.

Publication Date

4-2004

Publication Title

Applied and Environmental Microbiology

Volume

70

Issue

4

Start Page No.

2540

End Page No.

2544